Okay, these are my opinions on the mane 6's sexualities and genders. I had to delete this because of some enbyphobic comments, so this time, please read these articles about the gender spectrum before commenting.
http://sitn.hms.harvard.edu/flash/2016/gender-lines-science-transgender-identity/ -Written by Katherine J. Wu, a PhD Candidate of the Eric Rubin Lab at Harvard University. Statistics in this article were provided by Brad Wierbowski, a Research Assistant Graduate Student Cell Biology at Harvard university.
https://www.nationalgeographic.com/magazine/2017/01/how-science-helps-us-understand-gender-identity/ —By National Geographic.
https://erlc.com/resource-library/articles/what-science-really-has-to-say-about-sexuality-and-gender —By Joe Carter, a man with an MBA from Marymount University.
https://www.medicaldaily.com/challenging-gender-identity-biologists-say-gender-expands-across-spectrum-rather-323956 —By Samantha Olson, a woman who earned her BA in Professional Writing with a Business Administration minor at King’s College, and her MS in Journalism at Stony Brook University.
https://www.huffingtonpost.com/ravishly/neuroscience-proves-what_b_6494820.html- By Ravishly.